physio ex being unfaithful 0 workout 12 activity 1

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Disease

Experiment Benefits

Stop & Think Questions:

The source of the patient trials is

You appropriately answered: c. epithelial scrapings

Precisely what is the identity for the specific part of the antigen that the antibody binds to? You correctly answered: b. epitope

15. Move the 1st slide (patient A) to the fluorescent microscope. Count the number of elementary physiques you see through the microscope (recall that fundamental bodies discoloration green), enter the number of primary bodies during a call below, after which click Post Data to show off your brings about the main grid.

After you click Submit Data, the go will quickly be placed in the biohazardous waste disposal. You answered: 0 elementary physiques

16. Move the next go (patient B) to the fluorescent microscope. Rely the number of primary bodies the truth is through the microscope (recall that elementary systems stain green), enter the number of elementary bodies in the field below, and then just click Submit Data to display your results in the grid. When you click Post Data, the slide will certainly automatically always be placed in the

biohazardous waste disposal. You answered: 15 elementary body

17. Pull the next slip (patient C) to the fluorescent microscope. Count the number of general bodies you observe through the microscopic lense (recall that elementary body stain green), enter the number of elementary systems in the field listed below, and then click Submit Info to display the results in the grid. As soon as you click Send Data, the slide can automatically become placed in the biohazardous waste disposal. You responded: 1 primary bodies

18. Drag another slide (positive control) for the fluorescent microscope. Count the number of elementary physiques you see through the microscope (recall that elementary bodies spot green), enter the number of fundamental bodies during a call below, and then click Post Data to show your results in the main grid. After you simply click Submit Info, the slide will immediately be put in the biohazardous waste disposal. You answered: nineteen elementary body

19. Drag the last slip (negative control) to the fluorescent microscope. Rely the number of general bodies the thing is through the microscopic lense (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then just click Submit Data to display the results in the grid. Once you click Submit Data, the slide can automatically become placed in the biohazardous garbage disposal. You clarified: 0 fundamental bodies

Try things out Data:

Sample

Patient A

Sufferer B

Range of Elementary Body

zero

12-15

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Chlamydia Result

Negative

Positive

Sample

Patient C

Positive Control

Bad Control

Volume of Elementary Systems

you

nineteen

zero

10/15/13

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Chlamydia Result

Positive

Positive

Negative

Post-lab Quiz Outcomes

You scored 100% by addressing 5 away of five questions correctly. 1 . In the serology check just performed, the fluorescence is found within the You correctly answered: c. antibody

2 . What would probably happen in the event you forgot to repair the samples to the slip? You correctly answered: a. They would almost all test unfavorable because the washing steps will remove the trials. 3. Which sample revealed some left over non-specific holding after the cleansing steps? You correctly clarified: c. individual C

5. Which sufferer samples included the Chlamydia-specific antigen? You

properly answered: w. patient B

five. How can be chlamydia disease transmission eliminated?

You correctly clarified: d. celibacy or devoted, mutual monogamy

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Assessment Sheet Outcomes

1 ) Describe the value of the washing steps in the direct antibody fluorescence evaluation. Your solution:

necessary in order to take away any no specific binding that may occurred. 2 . Make clear where the epitope (antigenic determinant) is located. The answer:

On antigens because they are significant and have multiple antigenic sites”locations that can bind to antibodies. 3. Illustrate how a confident result is detected from this serological test out. Your solution:

When there is a presence of 10 or more fundamental bodies within a field diameter of five milimeters. They are going to stain green in reddish host cells.

four. How would the outcomes be damaged if a bad control offered a positive end result? Your solution:

It would be a “false negative and it would invalidate my results.

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